Figure 5

Induction of KLF2 mRNA by vesnarinone and downregulation of CXCR4 by KLF2. (a) Vesnarinone upregulates KLF2 mRNA in a time-dependent manner. B88 cells were treated with vesnarinone and quantitative RT-PCR was performed. (b) Forced expression of KLF2 downregulates CXCR4 mRNA. pcDNA3, KLF2 or ΔKLF2 expression vectors were transfected into B88 cells. After 48 h, quantitative RT-PCR was performed. (c) Forced expression of KLF2 impaired CXCR4 promoter activity. Stable transfectants of CXCR4 promoter-luciferase construct (-300/+86) were transfected with pcDNA3, KLF2, or ΔKLF2 expression vectors, and then luciferase activity was measured at the indicated time points. *, Significant as determined by one-way ANOVA (vs. pcDNA3, P < 0.01). (d) Forced expression of KLF2 impaired cell migration induced by SDF-1. B88 cells that had been transiently transfected with KLF2 expression vectors were seeded on the upper membrane in the presence or absence of SDF-1α in the lower compartment. After 24 h, cells attached to the lower surface of the membrane were stained by H&E and were counted. *, P < 0.05 (one-way ANOVA). The bars show the SD of triplicate samples. Data are representative of three separate experiments with similar results.