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Figure 3 | Molecular Cancer

Figure 3

From: Enhanced sensitivity of celecoxib in human glioblastoma cells: Induction of DNA damage leading to p53-dependent G1 cell cycle arrest and autophagy

Figure 3

Effect of celecoxib on p53-dependent cell cycle progression. Cells were synchronised in serum-free medium for 48 hours and then treated with celecoxib (8 and 30 μM) in medium containing 10% FBS for 18 hours. In some cases, U87MG cells were pre-treated with PFT (25 μM) prior to celecoxib treatment. Cells were collected for protein extraction and cell cycle analysis. Following release of cells from starvation, the effect of celecoxib on p53 protein expression in U87MG and PFT-treated U87MG cells is shown (A). For cell cycle analysis, cells were fixed, stained with propidium iodide and analysed by flow cytometry. Representative histograms of cell cycle progression (B-C) and quantitative analysis of percentage gated cells at G1, S and G2M phases (D-E) are shown. All values are expressed as mean ± S.E.M, n = 5–8.

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Molecular Cancer

ISSN: 1476-4598