Figure 5
From: STAT5 regulation of BCL10 parallels constitutive NFκB activation in lymphoid tumor cells
Y694F-mSTAT5A can localize to the nuclei of YT cells. (A) YT cells over-expressing vector alone (Vector), wt or Y694F mSTAT5A were stimulated with medium (-) or IL-2 (+) for 30 min at 37°C. Nuclear extracts were prepared and immuno-precipitated with anti-FLAG antibodies, resolved on 7.5% SDS-PAGE then Western blotted with PY antibodies followed by re-blotting with antibodies to STAT5 and FLAG as indicated to the right. (B) Nuclear extracts isolated as described above were resolved on a 7.5% SDS-PAGE, Western blotted with PY-STAT5 antibody then re-blotted with antibodies to STAT5, Lamin A/C and β-actin as indicated to the right.