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Table 1 Influence of supernatants from different neuroblastoma cell lines on endothelial cell growth and endothelial cell survival

From: Chemoresistance acquisition induces a global shift of expression of aniogenesis-associated genes and increased pro-angogenic activity in neuroblastoma cells

 

P-gp1

p53 mutation2

Endothelial cell growth (%)3

Endothelial cell survival (%)4

Positive control5

  

100 ± 8

100 ± 9

Negative control6

  

5 ± 7

12 ± 6

UKF-NB-3

-

-

15 ± 6

22 ± 8

UKF-NB-3rVCR10

+

+ (C135F)

78 ± 12*

83 ± 12*

UKF-NB-3rCDDP1000

-

-

89 ± 9*

85 ± 7*

UKF-NB-3rDOX20

+

-

66 ± 13*

70 ± 6*

UKF-NB-2

-

-

17 ± 11

20 ± 5

UKF-NB-2rVCR10

+

-

48 ± 13*

55 ± 8*

UKF-NB-2rCDDP1000

-

-

38 ± 7*

48 ± 10*

UKF-NB-2rDOX20

+

-

35 ± 9*

42 ± 9*

IMR-32

-

-

8 ± 6

17 ± 5

IMR-32rVCR10

+

-

63 ± 15*

67 ± 8*

IMR-32rCDDP1000

-

-

49 ± 7*

58 ± 6*

IMR-32rDOX20

-

-

37 ± 8*

44 ± 14*

UKF-NB-4

+

+ (C175F)

92 ± 9#

78 ± 15#

Be(2)-C

+

+ (C135F)

94 ± 16#

90 ± 7#

  1. 1 P-glycoprotein (P-gp) expression; + = overexpression, - = no overexpression
  2. 2 p53 status; + = mutated p53, - = wild-type p53
  3. 3 endothelial cells were grown in cancer cell culture supernatants mixed 1:1 with IMDM supplemented with 10% FCS; cell number was determined after 120 h
  4. 4 confluent endothelial cell monolayers were incubated with cancer cell culture supernatants mixed 1:1 with IMDM supplemented with 10% FCS; cell number was determined after 48 h
  5. 5 IMDM supplemented with 15% FCS, 5% human serum, bFGF 2.5 ng/ml
  6. 6 IMDM supplemented with 10% FCS
  7. * P < 0.05 relative to corresponding parental chemosensitive neuroblastoma cell line
  8. # P < 0.05 relative to the parental chmosensitive neuroblastoma cell lines UKF-NB-3, UKF-NB-2, and IMR-32.
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Molecular Cancer

ISSN: 1476-4598