Figure 5

Suppression of phosphorylation of Akt and c-Met as well as NF- κ B expression in MDA-MB-231 cells by BER. The cells were treated with BER at the indicated concentration and the 0 (vehicle) is the control. Equal amounts of protein from total cell lysates were separated by SDS-PAGE, and Western blot analysis of p-Akt (A), p-c-Met (C) and NF-κ B (B) were done. For the analysis of phosphorylation of Akt and c-Met, the density of the band (normalized to Akt and c-Met, respectively) shown as mean ± SD is relative to that of 0 (vehicle) as the control (designated as 100%). For analysis of the NF-κ B p65 expression, the density of the band (normalized to β-actin) shown as mean ± SD is relative to that of 0 (vehicle) as the control (designated as 100%). Bay and LY (LY294.002) are the inhibitors of NF-κ B and PI3K/Akt, respectively. For one experiment, 3 assays were carried out and only one set of gels is shown. The phosphorylation of Akt and c-Met as well as NF-κ B p65 expression in MDA-MB-231 cells were significantly suppressed by BER. Statistical analysis was carried out using the ANOVA and Bonferroni test. *: P < 0.05 (n = 3). Values with different letters (a-c) differ significantly (P < 0.05).