PCR artefacts detected in in a clinical FFPE sample (TX13). A total of 8 out of 31 LCN-HRM reactions were positive by melting curve analysis. Five non-identical sequence variants were detected from 4 of the 8 LCN-HRM positive reactions sequenced. In reaction 24, two transitional G>A changes were detected at positions c.2192 and c.2224. Two other exonic (c.2252C>T and c.2271G>T) and one intronic (c.2185-23T>C) variants were also detected in reactions 45, 57 and 25 respectively.