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Figure 4 | Molecular Cancer

Figure 4

From: Limited copy number - high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies

Figure 4

PCR artefacts detected in in a clinical FFPE sample (TX13). A total of 8 out of 31 LCN-HRM reactions were positive by melting curve analysis. Five non-identical sequence variants were detected from 4 of the 8 LCN-HRM positive reactions sequenced. In reaction 24, two transitional G>A changes were detected at positions c.2192 and c.2224. Two other exonic (c.2252C>T and c.2271G>T) and one intronic (c.2185-23T>C) variants were also detected in reactions 45, 57 and 25 respectively.

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Molecular Cancer

ISSN: 1476-4598