Figure 6
From: HIPK2 modulates p53 activity towards pro-apoptotic transcription
(a) RKO cells stable depleted of HIPK2 function (siHIPK2) were treated with ADR and zinc for 16 h in the presence of Nic or TSA. Gene expression of p53 targets was analysed by semiquantitative RT-PCR. GAPDH was used as a control for RNA extraction and transcription. (b) RKO cells HIPK2-depleted were treated as in (a) and apoptosis was measured by TUNEL assay 24 h after treatments and shown as percentage of TUNEL positive cells. The results shown are representative of two independent experiments performed in duplicate, ± S.D.