Figure 5
From: Canonical Wnt signaling is antagonized by noncanonical Wnt5a in hepatocellular carcinoma cells
Comparative analysis of Frizzled receptors and LRP co-receptors in hepatocellular carcinoma cell lines. Frizzled receptors involved in canonical and noncanonical Wnt signaling were tested for expression by RT-PCR assay (Top). The expression of LRP co-receptors was analyzed similarly (bottom). Total RNAs were extracted from cell lines and used to detect gene expression by RT-PCR assay. See Figure 1 for GAPDH loading control.