Aur-A down-regulates IκBα via Akt phosphorylation and induces p65 subunit of NF-κB nuclear translocation. (a) Cells were transiently transfected with Aur-A or pCS2+, or Aur-A siRNA or its scramble control. (b) Cells were incubated in serum-free media with VX-680 for 12 h. (c) Cells were treated with or without TNF-α 50 ng/ml, or transiently transfected with Aur-A or pCS2+, and subjected to immunofluorescence staining with anti-p65 antibody (green). DAPI (blue) was used to visualize the nuclei, original magnification × 1000. Histogram represented the percentage of cells with nuclear translocation from three independent experiments. (d) Aur-A or pCS2+, Aur-A siRNA or its control transfected cells treated with wortmannin 1 μM for 24 h before harvesting. (e) Cells were transfected with Aur-A or pCS2+ and treated with API-2 1 μM for 24 h prior to harvesting. (f) Cells were cotransfected Aur-A or pCS2+ with Akt1 siRNA or its control. Cell lysates were analyzed for indicated proteins by Western blot (a, b and d-f). (g) A diagram depicts the Aur-A-mediated pro-survival signaling pathways.