Figure 5
NF-κB regulates OPN-induced AP-1 activation. A. MCF-7 cells were transfected with pAP-1-Luc along with Renilla luciferase and then either treated with OPN alone or pretreated with anti-αvβ3 integrin blocking antibody along with OPN. In separate experiments, cells were transfected with IκBα super-repressor along with pAP-1-Luc and then treated with OPN. The transfection efficiency was normalized by Renilla and fold changes in luciferase activity with respect to control were calculated, and mean ± S.D. of triplicate determinations are plotted. The values were also analyzed by One Way ANOVA (*, p < 0.05; **, p < 0.05). B. MCF-7 cells were individually transfected with wild type and dominant negative c-Jun, wild type c-Fos, A-Fos and then treated with OPN for 30 min. Nuclear extracts were prepared and analyzed by EMSA. C. MCF-7 cells were individually transfected with wild type and dominant negative c-Jun, wild type c-Fos, A-Fos along with pNF-κB-Luc and then treated with OPN for 24 h. The transfection efficiency was normalized by Renilla and fold changes in luciferase activity with respect to control were calculated, and mean ± S.D. of triplicate determinations are plotted. The values were also analyzed by One Way ANOVA (*, p < 0.05; **, p < 0.05).