Figure 5

Effects of miR-101 on DU-145 and LNCaP cells. A and B. Effects of ectopic miR-101 on the proliferation and invasiveness of DU-145 cells. DU-145 cells were transfected by 120 nM of miR-cont or miR-101 mimics and cells were collected at 72 h post-transfection. Aliquots of the cells of each treatment were studied in triplicates by (A) WST-1 cell proliferation assay and (B) Matrigel invasion assay. Western blot analyses of these transfected cells are shown as an insert of "A" and representative images of invasion assay are also shown in "B" (right panel). C, and D. Effects of ectopic miR-101 on the morphology and proliferation of LNCaP cells. In C, the LNCaP cells at 72 h post transfection were imaged under microscope (20×). The black arrow heads indicate the rounding of the cell body, while the white arrow heads point the extensions of cell cytoplasmic portion. In D, the LNCaP cells were seeded in triplicate at a density of 6000 cells/well in 96-well plates and transfected with 120 nM of miR-cont or miR-101 mimics. Cell proliferation was studied by WST-1 reagent. E. Wound healing assay of LNCaP cells transfected with miRNA mimics (120 nM). Images were captured at the time points as indicated. F. Boyden chamber migration assay of LNCaP cells transfected with miRNA mimics (120 nM). The cell numbers were quantified after crystal violet staining with represented images shown below.