Figure 2
From: The anti-aging gene KLOTHO is a novel target for epigenetic silencing in human cervical carcinoma
Hypermethylation of the promoter region of KLOTHO in cervical cancer. A, Schematic representation of the upstream region of KLOTHO around the transcription start site (+1) and the primer location. The vertical lines represent the CpG dinucleotides. B, MSP assay of KLOTHO in cervical cancer cell lines. In vitro methylated DNA (IVD) from a placenta was used as a positive control for methylated DNA. C, Representative results of the MSP analysis of KLOTHO in human cervical tissue samples. Normal, LSIL, HSIL, and invasive carcinoma represent the histopathologic grades of cervical carcinogenesis.