Figure 4From: The anti-aging gene KLOTHO is a novel target for epigenetic silencing in human cervical carcinomaChIP analysis of the KLOTHO promoter region in SiHa cells. Antibodies against acetylated histone H3 (AcH3) and H4 (AcH4) were used to isolate acetylated chromatin fragments from lysates of control and TSA-treated SiHa cells, respectively. DNA fragments corresponding to the KLOTHO promoter around the transcription start site were amplified by PCR.Back to article page