Figure 3

Late apoptosis in HeLa, SiHa and HaCaT cells after in vitro exposure to Pentoxifylline (PTX) or Adriamycin (ADR) either alone or in combination. Cell cultures were treated with PTX (8 mM) or ADR (1 μM) or their combination (PTX + ADR), 24 hours later the cells were harvested and apoptosis was determined by UV light microscopy using ethidium bromide and acridine orange stains. The results represent the percentage of apoptosis ± standard deviation of three independent experiments carrier out in triplicate. Statistical analysis Student "t" test. (*) p < 0.001 vs untreated cells (CT).