Figure 7

Differential Inhibition of Thyroid Cancer Cell Line Invasiveness by Blocking NF-κB Signaling. BCPAP, SW1736, TPC1, and C643 cells were transduced with either Ad-GFP or Ad-mIκBα at an MOI of 200, 100, 50, and 100, respectively. After 24 hours, cells were serum-starved in RPMI (0.1% FBS) for 6 hours. Cells were then harvested by trypsinization and applied to Matrigel-coated transwell chambers in RPMI supplemented with 0.1% FBS. Invasion was promoted by the presence of RPMI supplemented with 10% FBS in the lower chamber. Invaded cells were then fixed, stained with DAPI, and counted, as described in Materials and Methods. Data is represented as percent cell invasion compared to Ad-GFP control (normalized to 100%), which is denoted by the dashed line. Mean ± S.E.M. of 3 independent experiments performed in duplicate is reported. [p < 0.05 (*); p < 0.01 (**); p < 0.001 (***)].