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Table 1 Relative quantitation of IL-6 expression in HA22T/VGH after arecoline treatment by quantitative real-time PCR method.

From: Arecoline induces HA22T/VGH hepatoma cells to undergo anoikis - involvement of STAT3 and RhoA activation

Arecoline (μg/ml) IL-6 GAPDH ΔCta ΔΔCtb related to controlc
0 28.70 ± 0.09 17.34 ± 0.07 11.36 ± 0.11 0 ± 0.11 1.00 (0.92-1.08)
3 28.67 ± 0.11 17.28 ± 0.06 11.39 ± 0.13 0.03 ± 0.13 0.98 (0.90-1.07)
30 29.11 ± 0.06 17.23 ± 0.08 11.88 ± 0.10 0.52 ± 0.10 0.70* (0.65-0.75)
100 31.54 ± 0.08 17.67 ± 0.03 13.87 ± 0.09 2.51 ± 0.09 0.18# (0.17-0.19)
  1. a: The ΔCt value is determined by subtracting the average GAPDH Ct value from the average IL-6 Ct value. All data are the mean ± standard deviation. b: The ΔΔCt value is determined by subtracting the control ΔCt value from the treatment ΔCt value. c: The range given for IL-6 relative to control is determined by evaluating the expression: 2(-ΔΔCt) with ΔΔCt+S.D. and ΔΔCt-S.D., where S.D. = the standard deviation of the ΔΔCt value. *: p < 0.05; #: p < 0.001 as compared to the untreated control.