Arecoline induces HA22T/VGH hepatoma cells to undergo anoikis - involvement of STAT3 and RhoA activation

Table 1 Relative quantitation of IL-6 expression in HA22T/VGH after arecoline treatment by quantitative real-time PCR method.

Arecoline (μg/ml)	IL-6	GAPDH	ΔCt^a	ΔΔCt^b	related to control^c
0	28.70 ± 0.09	17.34 ± 0.07	11.36 ± 0.11	0 ± 0.11	1.00 (0.92-1.08)
3	28.67 ± 0.11	17.28 ± 0.06	11.39 ± 0.13	0.03 ± 0.13	0.98 (0.90-1.07)
30	29.11 ± 0.06	17.23 ± 0.08	11.88 ± 0.10	0.52 ± 0.10	0.70* (0.65-0.75)
100	31.54 ± 0.08	17.67 ± 0.03	13.87 ± 0.09	2.51 ± 0.09	0.18^# (0.17-0.19)

^a: The ΔCt value is determined by subtracting the average GAPDH Ct value from the average IL-6 Ct value. All data are the mean ± standard deviation. ^b: The ΔΔCt value is determined by subtracting the control ΔCt value from the treatment ΔCt value. ^c: The range given for IL-6 relative to control is determined by evaluating the expression: 2^(-ΔΔCt) with ΔΔCt+S.D. and ΔΔCt-S.D., where S.D. = the standard deviation of the ΔΔCt value. *: p < 0.05; #: p < 0.001 as compared to the untreated control.

ISSN: 1476-4598