Figure 4

Real-time RT-PCR analysis of the host gene encoding miR-143 and - 145. Real-time RT-PCR analysis was performed on human normal tissues and cancer cell lines (A) and human colon adenocarcinoma samples (B) by using host gene-specific primers. Ten pairs of colon samples, including 10 human colon adenocarcinoma tissue samples and 10 matched normal colon tissue samples, were obtained from Osaka Medical College Hospital (Takatsuki, Osaka, Japan) and Fujita Health University Hospital (Toyoake, Aichi, Japan) with patients' informed consent. Collection and distribution of the samples were approved by each of the appropriate institutional review boards. The category of colon samples was confirmed by pathological analysis. Total RNA was extracted from the colon samples by using TRIzol (Invitrogen, Carlsbad, USA) according to the manufacturer's protocol. Expression levels of the host gene in each sample (LOC3 and LOC10, refer to Fig. 2) were detected by using a Superscript III reverse transcription kit (Invitrogen, Carlsbad, USA) and SYBR Premix Ex Taq (Takara, Otsu, Japan) and normalized with GAPDH. Ruled lines present the medians of samples. Data were analyzed by using Student's t-test. A value of p < 0.05 was considered significant.