Skip to main content
Figure 19 | Molecular Cancer

Figure 19

From: Constitutively overexpressed 21 kDa protein in Hodgkin lymphoma and aggressive non-Hodgkin lymphomas identified as cytochrome B5b (CYB5B)

Figure 19

Quantification of CYB5B RT-PCR data from clinical samples. cDNA was prepared from both CD3-selected and CD19-selected cells from normal peripheral blood mononuclear cells (PBMNC), reactive tonsils, normal bone marrow (BM) and reactive lymph node samples. PCR was performed using primers to the GAPDH housekeeping gene. Bands from the GAPDH PCR were used to normalize loading for the CD3+ and CD19+ cDNA pools for each specimen. Then, PCR was performed in duplicate for CYB5B and GAPDH, using the same amount of cDNA and the same number of cycles. Bands were quantified using spot densitometry and the ratio of CYB5B/GAPDH signal was calculated. Values shown are from a typical experiment; each sample was analyzed by at least two independent PCRs. In none of the samples did the CYB5B signal exceed that of GAPDH.

Back to article page

Molecular Cancer

ISSN: 1476-4598