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Figure 6 | Molecular Cancer

Figure 6

From: Constitutively overexpressed 21 kDa protein in Hodgkin lymphoma and aggressive non-Hodgkin lymphomas identified as cytochrome B5b (CYB5B)

Figure 6

Protein array identification of the molecular target of R23.1. Panel A: A PEX protein array containing approximately 16,000 bacterial clones expressing human recombinant His-tagged proteins was screened with the antibody R23.1. The antibody recognised a single clone, 9028C1242. Panel B and C: The clone 9028C1242 (full length CYB5B) and the clone 9028A0834 (expressing the COOH terminal region of CYB5B) were expressed and Western immunoblotted with both R23.1 and an anti-His antibody. R23.1 clearly recognises the approximately 20 kDa full length CYB5B, but not the COOH-region of the protein, revealing the epitope of the antibody to lie within the first 70aa.

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Molecular Cancer

ISSN: 1476-4598