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Figure 3 | Molecular Cancer

Figure 3

From: The chemopreventive retinoid 4HPR impairs prostate cancer cell migration and invasion by interfering with FAK/AKT/GSK3β pathway and β-catenin stability

Figure 3

4HPR antagonizes AKT activation by IGF-I but not constitutively active AKT. A). DU145 cell migration towards FB-CM is significantly enhanced by IGF-I (100 ng/ml), as compared to control cells. IGF-I effects are completely abrogated when cell migration is carried out in the presence of 4HPR (5 μM). Experiments were done in triplicate and repeated thrice. Means ± SD are shown (*P < 0.05, ***P < 0.001). B). Western blot analysis of extracts from DU145 cells pulsed for 30 min with IGF-I at 100 ng/ml show strong AKT phosphorylation, almost completely abolished by 4 h pretreatment with 5 μM 4HPR. C). DU145 cells transiently transfected with a constitutively active form of AKT (Myr.Akt) and showing high levels of pAKT as compared to vector transfected control cells (inset) are no more susceptible to 4HPR-induced inhibition of cell migration. PC3 transfected cells produced similar results.

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Molecular Cancer

ISSN: 1476-4598