Figure 6
Confirmation of the microarray gene expression for six randomly selected significantly regulated genes in rat early HCC by realtime qRT-PCR. Ratio of expression (fold change) for each gene in (A) early HCC in young (DY) compared to normal (NY); (B) DY group to regenerated (RY), (C) early HCC in old (DO) compared to normal (NO); (D) DO group to regenerated (RO). A significant correlation existed between the microarray and realtime RT-PCR results (p < 0.001), thus demonstrating the reliability of our gene expression measurements. The fold changes were log2 transformed for both microarray data and real-time RT-PCR. Grey bars represent microarray hybridizations, and, and dark bars represent values from qRT-PCR. The error bar represents standard deviation (SD) over four experiments. P-values for triplicate analyses were all < 0.05.