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Figure 2 | Molecular Cancer

Figure 2

From: Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia

Figure 2

Detection of AGR2 by Western blot with mAb AGR2.43. A, Western blot of lysates from the human pancreatic cancer cell lines CFPAC-1 and MiaPaca, recombinant GST-tagged AGR2 (rAGR2) and AGR3 (rAGR3). As expected, mAb AGR2.43 detected the AGR2 protein (20 kDa) in CFPAC-1, but not MiaPaca lysates. The mAb also detected rAGR2 (45 kDa) but not rAGR3 (45 kDa). Note that rAGR2 and rAGR3 are of a higher molecular weight than their native counterparts because they carry a GST tag. B, Coomassie stained gel showing equivalent loading (1 μg each) of GST-tagged rAGR2 and rAGR3 proteins.

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Molecular Cancer

ISSN: 1476-4598