Figure 3

AGR2 ELISA standard curves. rAGR2 was diluted in either 1× Reagent Diluent (RD) or RD supplemented with 10% normal human serum (RD+10%NHS). A 3-fold dilution series of rAGR2 (from 40 ng/ml to 0.006 ng/ml) was generated in RD or RD+NHS. AGR2 ELISA was used to measure the signal for each sample in RD (closed circles) or RD+10%NHS (closed squares). Standard curves were generated for AGR2 in RD (solid line) or RD+10%NHS (dashed line) using the five-parameter log-logistic method (r² = 0.999 for both curves). The average of three replicates is presented. Error bars represent one standard deviation and are almost not apparent due to low intra-assay standard deviations.