Figure 2
MUC1 expression prevents and MUC1-galectin-3 interaction promotes homotypic aggregation of MUC1 positively but not negatively transfected human breast epithelial cells. A: Representative flow cytometry plots from the aggregation assessment of MUC1 positive-transfectants (HCA1.7+) and negative-revertants (HCA1.7-) of HBL-100 human breast epithelial cells in the presence or absence or 1 Ī¼g/ml recombinant galectin-3. B: HCA1.7+ cells show less spontaneous cell-cell aggregation than HCA1.7- cells. Data are expressed as mean Ā± SEM of triplicate determinations from four independent experiments. C: Galectin-3 (1 Ī¼g/ml) increases HCA1.7+ but not HCA1.7- cell aggregation. Data are expressed as mean Ā± SEM of triplicate determinations from four independent experiments. D: Galectin-3 induces dose-dependent aggregation of HCA1.7+ cells. Data are expressed as mean Ā± SEM of triplicate determinations from three independent experiments. E: The presence of lactose (10 Ī¼M) blocks the increase of HCA1.7+ cell aggregation induced by 1 Ī¼g/ml galectin-3. Data are expressed as mean Ā± SEM of triplicate determinations from two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.