Figure 5

MCF10A cells overexpressing the ∆PS mutant of BMI1 (MCF10A-BMI1∆PS) exhibit an invasive phenotype in 3-D Matrigel culture. MCF10A-B0, MCF10A-BMI1WT and MCF10A-BMI1∆PS cells were cultured in chamber slides in 2% Matrigel for 12 days and studied for Matrigel phenotype. Top panel shows the phase contrast images of the acini at day 12. At day 12, the cultures were fixed and immunostained using antibodies specific for E-cadherin (middle panel) and anti-α6 integrin (bottom panel). The nuclei were stained with Topro-3. After staining, acini were photographed (40X) using a Nikon Eclipse 80i confocal microscope.