Figure 4
From: EGFR isoforms and gene regulation in human endometrial cancer cells
Immunoblot Analysis of EGFR Isoform Expression in Ishikawa H and Hec50co Cells. Cell lysates from endometrial or transfected control (CHO-EGFR, CHO-sEGFR) cell lines were resolved by SDS-PAGE (7.5% acrylamide), and immunoblotted using enhanced chemiluminescence with antibodies specific for either: A) EGFR (isoform A, p170, intracellular domain), or B) sEGFR (isoform D, p90/110). Anti-tubulin labelling is included as a loading control (lower panel, Figs. A and B). Molecular weight markers migrate at the positions indicated: 250 kD, 150 kD, and 100 kD.