Figure 1

Hsa-mi R-124 methylation in primary keratinocytes (EK cells) and cervical cancer cell lines SiHa, CaSki and HeLa. A. hsa-miR-124-1 methylation, B. hsa-miR-124-2 methylation, C. hsa-miR-124-3 methylation. Whereas in primary keratinocytes no methylation was detectable, all cervical cancer cell lines were positive for methylation of hsa-miR-124-1, hsa-miR-124-2 and hsa-miR-124-3. D. In SiHa cells treated with 5000 nM DAC, methylation levels of hsa-miR-124-1 (black), hsa-miR-124-2 (white) and hsa-miR-124-3 (grey) were reduced by more than 50%. Methylation levels of all regions in untreated cells were set to 100%. E. Whereas in untreated and mock (PBS) treated SiHa cells no hsa-miR-124 expression was detectable, SiHa cells treated with 5000 nM DAC showed clear hsa-miR-124 expression.