Hsa-miR-124 methylation and expression in early and late passages of HPV16 (FK16A/FK16B) and 18 (FK18A/FK18B) immortalised keratinocytes. A. hsa-miR-124-1, B. hsa-miR-124-2. Both hsa-miR124-1 and hsa-miR-124-2 showed little to no methylation in early passages and increasing levels of methylation in later passages of HPV16 and HPV18 immortalised cells. Note the difference in scales of Figure 2A and 2B. Levels of hsa-miR-124-1 methylation in HPV-immortalised keratinocytes are very low compared to the levels seen in cervical cancer cell lines (Figure 1A). No methylation of hsa-miR-124-3 was found in HPV-immortalised keratinocytes. C. Late passages of FK16B and FK18B cells showed reduced expression of hsa-miR-124 compared to their corresponding earlier passages.