Table 1 Sequences of qMSP and qRT-PCR primers used in this study
From: Methylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer
Gene | Primers(5'-3') | Size (bp) | Annealing (°C) |
|---|---|---|---|
hsa-miR-124-1 | F: CGGCGGGGAGGATGTT | 58.9 | |
R: ATAAAAAACGACGCGTATACGTACG | 94 | 59.4 | |
P: CGGCGTTTTTTATTTTT-Xsprobe | 70.0 | ||
hsa-miR-124-2 | F: GGGTAATTAATTTGGATTTACGTCGTTAT | 59.9 | |
R: CGTAAAAATATAAACGATACGTATACCTACGT | 138 | 58.8 | |
P: TTTACAACACACGCCTAAA -Xsprobe | 69.0 | ||
hsa-miR-124-3 | F: ACGCGGCGAAGACGTTT | 59.0 | |
R: CGAACGACGAACGTCGAAA | 95 | 59.4 | |
P: AAAATCCTCGCCCGAAAAACGCGA | 70.4 | ||
IGFBP7 | F: CCCAGGTGTACTTGAGCTGTGA | 89 | 58.9 |
R: TGAACTCCATAGTGACCCCTTTTT | 58.9 | ||
snRNP U1A | F: TCCTCACCAACCTGCCAGA | 71 | 58.8 |
R: TGAAGCCAGGGAACTGATTGA | 59.3 |