Figure 5
From: Characterizing cancer cells with cancer stem cell-like features in 293T human embryonic kidney cells
EMT and pro-metastatic genes are upregulated in 3D spheres of 293T cells. A) 293T spheres show upregulation of markers of the mesenchymal phenotype in the epithelial-mesenchymal transition such as higher n-cadherin, vimentin, and zeb-1 and low E-cadherin compared to 2D cells. B) RT-PCR demonstrated higher expression of EMT promoting genes Snail and Slug in 3D sphere culture vs. 2D monolayer culture. C) Western blot demonstrated upregulation of known proteins important in promoting metastasis such as RhoC, Tenascin-C and MTA1 in 3D spheres compared to 2D culture.