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Figure 3 | Molecular Cancer

Figure 3

From: Cadmium down-regulates expression of XIAP at the post-transcriptional level in prostate cancer cells through an NF-κB-independent, proteasome-mediated mechanism

Figure 3

Cadmium blocks expression of various XIAP domains. (A) PC-3 cells were transfected with the indicated HA-tagged XIAP constructs or (B) BIR 1-2 constructs with point mutations in zinc binding sites under the control of the SV40 promoter. Four hours after transfection cell culture medium was replaced with medium containing cadmium (30 μM) and cells were cultures for an additional 16 hours. Expression of XIAP truncated mutants and α-actin was detected by immunoblotting with anti-HA or anti-actin antibodies, respectively. Representative data from one of three experiments.

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Molecular Cancer

ISSN: 1476-4598