Figure 2

GFPdnLMP1 inhibits the clonogenicity of PyLMP1 transgenic carcinoma cell lines. Cell lines 53.217 (transgene negative control) and 53.234a (PyLMP1 transgenic) were transfected with 5 μg of either pGFP or GFPdnLMP1 or mock transfected (no DNA) in duplicate. After 24 hrs cells were passaged 1 to 8 and cultured for two weeks under G418 selection before fixing and staining with crystal violet. Colony numbers for these and further cell lines treated in the same way are given in Table 1.