Figure 3
From: ΔNp63 transcriptionally regulates ATM to control p53 Serine-15 phosphorylation
ΔNp63 isotypes stimulate ATM-dependent phosphorylation. (A) p63 controls clonogenic cell growth in tumour cells. H1299 cells were transfected with 1 μg p63 plasmids TAα, TAγ, ΔNα, or ΔNγ, or empty vector control, colonies were giemsa stained after 14 days selection and counted. (B) ΔNp63 isoforms stimulate p53 Serine-15 phosphorylation. H1299 cells were cotransfected with 0.2 μg p53 and 0.5 - 1 μg p63 variant as indicated and harvested after 48hrs. Lysates were blotted for the indicated proteins. (C) TAp63 isoforms induce p21WAF1 protein expression. H1299 cells were transfected with 1 μg p53, E2F-1, p63 isoforms TAα, TAγ, ΔNα, or ΔNγ, or empty vector control, and harvested after 48 hours. Lysates were immunoblotted for the indicated proteins.