Figure 5

The CCAAT element mediates ΔNp63α- and E2F-1-dependent stimulation of the ATM promoter. (A) Schematic representation of the human ATM promoter organisation (Genebank U82828), showing the position of putative response elements which are mutated in this study. (B) The CCAAT sequence mediates ΔNp63α and E2F-1 stimulated ATM transcription. H1299 cells were co-transfected with 1 μg ΔNp63α respective with 1 μg E2F-1, 1 μg ATM-LUC (wt or CCAAT mutant) and 0.2 μg pRL-CMV control plasmids, then lysed and processed after 24 hrs. Specific ATM-LUC activity was determined as described in Figure 4 throughout, and data is normalised to wild-type reporter activity. (C) ΔNp63α and E2F-1 have additive effects on ATM promoter stimulation. H1299 cells were transfected with 0.5 μg ΔNp63α, E2F-1 or 0.5 μg each plasmid along with 1 μg ATM-Luc (wt or mutant) and 0.2 μg pRL-CMV control plasmids, and cells were harvested and processed after 24 hrs. Total DNA was balanced with empty pCDNA3.1 vector.