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Figure 4 | Molecular Cancer

Figure 4

From: Tetrathiomolybdate inhibits head and neck cancer metastasis by decreasing tumor cell motility, invasiveness and by promoting tumor cell anoikis

Figure 4

TM treatment significantly inhibits oral squamous cell motility. A-B; OSCC-3 cells were cultured on top of a field of microscopic fluorescent beads in the following culture conditions: in the absence of serum and TM (S-TM-); in the presence of serum but no TM (S+TM-); in the absence of serum but presence of TM (S-TM+) or in the presence of serum and TM (S+TM+). After a 16-hours incubation period, cells were fixed and areas of clearing in the fluorescent bead field corresponding to phagokinetic cell tracks were quantified using NIH ScionImager. C; LOX catalytic activity was measured in whole-cell lysates of untreated (NT) or TM treated OSCC-3 cells (30 minutes). *, represents a significant difference (p < 0.05) as compared to the control group. D; Whole cell lysates of untreated or TM treated OSCC-3 cells were separated using 4-12% NuPAGE Bis-Tris gels and probed with anti-phospho-FAK antibody. Equal sample loading was verified by stripping the blots and re-probing with anti-tubulin antibody.

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