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Figure 2 | Molecular Cancer

Figure 2

From: R-Roscovitine (Seliciclib) prevents DNA damage-induced cyclin A1 upregulation and hinders non-homologous end-joining (NHEJ) DNA repair

Figure 2

Nuclearization of cyclin A1 under DNA DSB conditions and its role in NHEJ. A) Immuno-fluorescence analysis by fluorescent microscopy of cyclin A1 localization in A549 cells after treatment with Doxorubicin (750 nM and 2.5 μM). Lower panels show FITC-stained cyclin A1 expression (green) and upper panels show FITC and DAPI (blue) merge at 400× magnification. B) Fold change, respect to YFP, of in vitro NHEJ plasmid re-ligation activity as quantified by real time PCR in HEK293FT cells transiently transfected with YFP (control) or cyclin A1 (CCNA1) and respective western blot and ponceau S staining verifying overexpression respect to Hsp70.

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Molecular Cancer

ISSN: 1476-4598