Molecular characterization and the effect of TCM on proliferation of 10T1/2 mesenchymal stem cells. A. Identification of mesenchymal properties of 10T1/2 cells by determining the expression of epithelial or mesenchymal marker proteins using Western blot analysis. B. A comparative study of α-smooth muscle actin (α-SMA) and desmin expression level in 10T1/2 cells and AOSMC by Western blot analysis using specific antibodies. The bar graphs represent the quantitative data (mean ± SEM) from three separate experiments. P-values were determined by unpaired student's t-test. **p < 0.005 vs 10T1/2 and ***p < 0.001 vs 10T1/2. C. Detection of α-SMA and calponin in 10T1/2 cells and AOSMC using immunofluorescence. D. Effects of different TCM on 10T1/2 cell proliferation. 1, regular media (RM), 2, MCF-7 cells derived conditioned media, 3, MDA-MB-231 cells derived conditioned media and 4, Mia-Paca-2 cells derived conditioned media. P-values were determined by unpaired students't-test. *p < 0.01 vs RM; #p < 0.05 vs MCF-7-CM.