NRP-1 is required for PDGF-B mediated induction of α-SMA and desmin expression. A. Representative immuno-Western blot shows the effect of MCF-7-CM on NRP-1 expression in 10T1/2 cells. 1, RM and 2, MCF-7-CM. *p < 0.001 vs RM. B. Detection of the effect of NRP-1 antibody on PDGF-B-induced Desmin and α-SMA expression in 10T1/2 cells. The bar graphs represent quantification of the Desmin and α-SMA of three different experiments, P-values were determined by unpaired students't-test. *p < 0.001 vs controls; **p < 0.001 vs PDGF treated samples. Ns, non-significant with controls.