Comparison of L3MBTL4 mRNA levels in deleted and non deleted breast tumors. mRNA levels were measured by quantitative RT-PCR. GUSB mRNA expression was used as internal control for mRNA normalization. L3MBTL4 mRNA expression in deleted tumors was lower than in normal breast (NB). Among the non-deleted tumors, L3MBTL4 mRNA expression was lower in luminal than in normal breast (NB) and basal tumors. This suggests that the L3MBTL4 gene expression can be affected by heterozygous deletion but also regulated by an epigenetic mechanism. Asterisk (*) indicates the mutated samples.