STK10 and TNK2 knockdown induces apoptosis in Ewing's sarcoma cells. TC-71 cells were transfected with siRNAs against STK10, TNK2 or non-silencing control. (A) STK10 and TNK2 knockdown by siRNA induces apoptosis in Ewing's sarcoma cell lines. Bar graph showing the increase in annexin V staining in Ewing's sarcoma cells treated with siRNA targeting STK10 and TNK2 compared to non-silencing control siRNA. Number of cells labeled with annexin V over total number of cells as determined by Hoechst staining from three image fields is shown above the bar for each siRNA treatment. (B) Representative image showing Hoechst stained nuclei of TC-71 cells treated 72 hours with STK_6 siRNA and corresponding Annexin V stained image.