Figure 2
From: A Pleiotrophin C-terminus peptide induces anti-cancer effects through RPTPβ/ζ
P(122-131) inhibitory effect is attributed to its specific amino acid sequence and charge. (A) Number of adherent DU145 and LNCaP cells in the presence of 20 μM P(122-131), AAD, or 5K, as determined by the crystal violet assay. (B) Soft agar growth assays showing anchorage-independent proliferation. (C) Migration of cells through Transwell filters. (D) Effect of 2 nmol P(122-131), AAD, or 5K on angiogenesis as measured by the chicken embryo CAM assay. Results are mean values ± SE from at least 3 independent experiments.