Figure 1

RhoH regulates IL3-induced proliferation. (A) RhoH mRNA expression levels in control cells, RhoH or siRhoH-transduced BaF3 cells were detected by quantitative real-time PCR using RhoH specific primers and GAPDH as a reference gene. Statistical significance was analysed using the 's t-test (mean ± SD, n = 2 and *P ≤ 0.05; **P ≤ 0.01). (B) FACS analysis of control cells transduced with the empty vector (pMX-IRES-CD4-Puro) or RhoH (pMX-IRES-CD4-Puro RhoH) -transduced BaF3 cells for human CD4 expression after puromycin selection. Parental BaF3 cells were used as a negative control. (C) Dose response curve of control cells, RhoH and siRhoH BaF3 cells cultivated with increasing concentrations of IL3. Viability was determined by quantification of cellular ATP in a Cell Titer Glo assay. Results shown are from one representative experiment performed in triplicates ± SD. (D) Dose response curve of BaF3 cells, BaF3-EpoR cells and BaF3-EpoR cells overexpressing RhoH cultivated with increasing concentrations of Epo. Viability was determined by quantification of cellular ATP in a Cell Titer Glo assay. Results shown are from one representative experiment performed in triplicates ± SD.