Figure 4
From: I-kappa-kinase-2 (IKK-2) inhibition potentiates vincristine cytotoxicity in non-Hodgkin's lymphoma
ML120B:vincristine combination induces apoptosis in G2/M phase cells. WSU-FSCCL cells were pretreated with ML120B (M) for 60 minutes followed by vincristine (V) at indicated concentrations in Panel B then analyzed for Terminal transferase dUTP Nick End Labeling (TUNEL) staining using flow cytometry. A. Representative histograms using the higher concentrations of M and V; upper panel: untreated, control cells; lower panel: treated with ML120B (40 μM) and vincristine (520 pM). B. Full data of FITC positive cells by cell cycle phase (sub G0, G0/G1, S, G2/M) according to PI staining of DNA. The largest FITC positive population in treated cells was in G2/M followed by sub G0.