Figure 7

Immunofluorescence microscopy images of WSU-FSCCL cells. Top panel represents control cells after 48 h of culture; top left, tubulin staining; top center, NF-κB p65; top right, overlay of tubulin and p65. Bottom panel represents images of WSU-FSCCL cells after 48 h of treatment with ML120B (40 μM) and vincristine (520 pM). ML120B was added 60 minutes prior to vincristine: Bottom left are vincristine-alone treated cells showing disruption of microtubules and translocation of p65 to the nucleus as indicated by yellow-orange color. Bottom center: ML120B plus vincristine treatment showing sequestration of p65 in the cytoplasm in most cells. Bottom right: is same as bottom center except with DAPI counterstain to demarcate the nuclei and confirm absence of p65 from nuclei in most cells.