Figure 1
From: MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma
As-miR-221/222 increases glioma apoptosis. (A) Identification of differential expression of miR-221/222 in glioma cell lines (1, A172; 2, TJ905; 3, TJ899; 4, U251; 5, TJ866; 6, H4; 7, LN229) by Northern blot analysis. (B) U251 and LN229 cells were transfected with As-miR-221/222, and miR-221/222 expression level was detected by Northern blot assay. U6 was regarded as endogenous normalizer. (C) Annexin V analysis showed that U251 and LN229 cells transfected with As-miR-221/222 displayed significantly more apoptosis than the other four groups. (D) U251 and LN229 cells were transfected with As-miR-221/222, and Bax and Bcl2 protein level was detected by Western blot assay. β-actin protein was regarded as endogenous normalizer. (E) A significant increase in caspase 3/7 activity was detected in U251 and LN229 cells transfected with As-miR-221/222. (F) U251 cells transfected with As-miR-221/222 showed a significantly greater collapse in mitochondrial membrane potential compared with the other four groups. * P < 0.05 compared with control group, ** P < 0.01 compared with control group.