Enforced expression of ectopic FLIP
attenuates the effects of the GGTI-298 and TRAIL combination on decreasing cell survival ( A ) and inducing apoptosis ( B ). A, The indicated transfectants were seeded in 96-well plates and treated with the indicated concentrations of GGTI-298 plus 20 ng/ml TRAIL. After 24 h, the cells were subjected to the SRB assay for measurement of cell number. Data are means of four replicate determinations. Bars, ± SDs. B, The indicated transfectants were treated with DMSO, 15 μM GGTI-298, 20 ng/ml TRAIL, and the combination of GGTI-298 and TRAIL for 24 h and then subjected to detection of apoptosis by Annexin V staining. GGTI, GGTI-298.