Figure 3From: Coordination of glioblastoma cell motility by PKCιEffects of PKCι pseudosubstrate inhibitor peptide on cell motility. A. U87MG cells were treated with 20 or 50 μM atypical PKC pseudosubstrate peptide inhibitor (PSI) for 2 h. Whole cell lysates were then analyzed for levels of phosphorylated PKCι and total PKCι by Western blotting. B. Migration distance per minute (um/min) was quantitated using Ziess LSM image browser software. Bar graphs show the mean +/- SD from three independent videomicroscopy experiments each for control and PS-I treated U87MG cells (mean of 10 cells quantified per experiment). C. Quantitation of leading edge formation from videomicroscopy. Cells from three independent movies were analyzed for the formation of a single dominant leading edge. Bar graphs show the mean +/- SD. D. Representative examples comparing cell motility in control cells and cells treated with 20 μM PS-I. Still images from videomicroscopy are shown, with times indicated on the left. A representative full video is shown in Supplementary video S3.Back to article page