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Figure 1 | Molecular Cancer

Figure 1

From: Withanolide D induces apoptosis in leukemia by targeting the activation of neutral sphingomyelinase-ceramide cascade mediated by synergistic activation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase

Figure 1

Anti-proliferative effect of WithaD. (A) Chemical structure of WithaD. (B) Morphological changes induced by WithaD in K562, MOLT-4 and Vero cells, as demonstrated by phase contrast microscopy. (C) Effect of WithaD (0-5 μM) on the cell viability of K562 and MOLT-4 cells after 24 and 48 hr as demonstrated by MTT assay. (D) Normal lymphocytes were isolated form normal blood, cultured for 48 hr in the presence of WithaD at concentration ranged from 0-20 μM and checked the cell viability by trypan blue dye exclusion assay. (E) Flow cytometric analysis revealed in situ DNA fragmentation by TUNEL assay in WithaD treated MOLT-4 (0.5 μM) and K562 (1.5 μM) cells at indicated time. The plots are representative of three independent experiments. The percentages of TUNEL positive cells were indicated in respective panel.

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Molecular Cancer

ISSN: 1476-4598