Table 1 Primer sequences
Primer | Primer sequence | Fragment Position | Primer Length (bp) | Tm (°C) |
|---|---|---|---|---|
Ceramide synthase (LASS2-001), size: 242 bp | ||||
Sense Primer | 5'-TTCTTTGAGCTGTACGTGGCTA-3' | 549-571 | 22 | 60.07 |
Antisense primer | 5'-TCTCGGAACTTCTTGAGGAGAC-3' | 790-768 | 22 | 59. 99 |
Acid sphingomyelinae (A-SMase), size: 146 bp | ||||
Sense Primer | 5'-GAGTAGAGGCCTAAGTTGAC-3' | 2181-2201 | 20 | 50.74 |
Antisense primer | 5'-GGAGTCCAAGTCTCTTATCT-3' | 2326-2346 | 20 | 49. 90 |
Neutral sphingomyelinase (SMPD2) size: 240 bp | ||||
Sense Primer | 5'-ACAATCGACAGAAGGACATC-3' | 781-801 | 20 | 55.00 |
Antisense primer | 5'-AGTTCTTGGGTACCATTGTG-3' | 1020-1000 | 20 | 54. 99 |
Neutral sphingomyelinase (SMPD3) size: 296 bp | ||||
Sense Primer | 5'-TGTTACCCCAACAAGTGTAACG-3' | 1681-1703 | 22 | 59.82 |
Antisense primer | 5'-TCGTCAGAGGAGCAGTTATCAA-3' | 1976-1954 | 22 | 60.02 |
Neutral sphingomyelinase (SMPD4) size: 177 bp | ||||
Sense Primer | 5'-ATCCTGTGGAGTACAGCATC-3' | 864-884 | 20 | 55.04 |
Antisense primer | 5'-TGTGGTACAGAGGACTGTCA-3' | 1040-1020 | 20 | 54.89 |
β-actin, size:137 bp | ||||
Sense Primer | 5'-CGACAGGATGCAGAAGGAG-3' | 1014-1032 | 19 | 62 |
Antisense primer | 5'-ACATCTGCTGGAAGGTGGA-3' | 1150-1132 | 19 | 58 |