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Figure 3 | Molecular Cancer

Figure 3

From: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway

Figure 3

DIF-1 inhibited in vitro angiogenesis. (A) DIF-1 inhibited tube formation of HUVECs. HUVECs were seeded in Matrigel-coated well with or without DIF-1 30 μM and incubated for 8 h. The tube formation of control HUVECs was normalized as 100%. Each value represents the mean ± SE of three independent experiments. The asterisk indicates *P < 0.01 versus control. The scale bars indicate 200 μm. (B) DIF-1 inhibited the migration of HUVECs. HUVECs were seeded into the upper part of Boyden chamber. The lower compartments were filled with 600 μl of DMEM supplemented with 0.1% bovine serum albumin and 20 ng/ml (0.52 nM) VEGF. After HUVECs were incubated for 10 h, the migrated cells on the lower surface of the membrane were quantified by counting the number of cells in ten random fields per membrane and expressed as cells/fields (mean ± SE). Data are representative of three other experiments. The asterisk indicates *P < 0.01 versus control. The scale bars indicate 500 μm.

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Molecular Cancer

ISSN: 1476-4598